METHOD FOR PRODUCING PREPARATIONS OF ISOLATED CELLS Russian patent published in 1998 - IPC

FIELD: cytology for cytologic study of processes involved in histogenetic development of various tissues, study of cytophysiological laws governing hypertrophic and atrophic processes. SUBSTANCE: preparations of only those of cells are produced which must be studied. For this purpose tissues fixed with formalin for 14-20 days are subjected to freezing. Fragment is isolated from prescribed tissue components of organs taken in the form of cuts 20-40 mcm thick, followed by dissociating fragment thus-isolated in 50-% KOH for 2.5-3 h at temperature 18-20 C. Fragment is washed five times with water at 18-20 C, each for 5 min. EFFECT: method it possible to achieve high-precision isolation of microfragments from tissues of various organs, thereby enabling selective analysis of dissociated cells making part of only tissue structures to be studied. 1 dwg