STRAIN OF BACTERIUM STREPTOMYCES SPECIES SJ-12 - PRODUCER OF RESTRICTION ENDONUCLEASE RECOGNIZING AND SPLITTING NUCLEOTIDE SEQUENCE 5'-CTGCAG-3' Russian patent published in 1999 - IPC

Abstract RU 2135581 C1

FIELD: biotechnology, genetic engineering. SUBSTANCE: strain Streptomyces sp. SJ-12 - a producer of restrictase Ssp12-1 is isolated from the soil as a result of restrictase producers screening. Invention provides preparing the restrictase enzyme that recognizes and splits the nucleotide sequence 5'-CTGCAG-3'. The yield of enzyme is 50 000 U/g wet biomass, specific activity of the end enzyme is 50 000 U/ml. Restrictase Ssp12-1 is an isoschizomer of restrictase Sal PI and can replace the latter in all genetic engineering investigations. EFFECT: strain and enzyme indicated above, high yield and activity of enzyme. 1 dwg, 2 tbl, 4 ex

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RU 2 135 581 C1

Authors

Puchkova L.I.

Andreeva I.S.

Ushakova T.A.

Krivopalova G.N.

Repin V.E.

Dates

1999-08-27—Published

1997-09-26—Filed