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(19)

(11)

2 302 459

(13)

(51)

IPC

C12N1/20(2006-01-01)

C12R1/06(2006-01-01)

(21) (22)

Application

2005129658/13, 2005-09-22

(24)

Start date

2005-09-22

(22)

Actual filing date

2005-09-22

(45)

Published

2007-07-10

(72)

Inventor

Puchkova Larisa IvanovnaTotmenina Ol'Ga DmitrievnaAfonina Veronika SergeevnaPolushkina Alla FridrikhovnaRepin Vladimir Evgen'Evich

(73)

Holder

Federal'Noe Gosudarstvennoe Uchrezhdenie Nauki Nauchnyj Tsentr Virusologii I Biotekhnologii Federal'Noj Sluzhby Po Nadzoru V Sfere Zashchity Prav Potrebitelej I Blagopoluchija Cheloveka

BACTERIAL STRAIN OF Arthrobacter species Mn372 AS PRODUCER OF Asi3721 RESTRICTION ENDONUCLEASE RECOGNIZING AND CLEAVING OF 5'-ATGCAT-3' NUCLEOTIDE SEQUENCE Russian patent published in 2007 - IPC C12N1/20 C12R1/06

Abstract RU 2302459 C2

FIELD: microbiology, gene engineering.

SUBSTANCE: Arthrobacter species Mn372 strain id producer of Asi3721 restriction endonuclease representing isoshisomer of Ava III restriction endonuclease. Method of present invention makes it possible to produce restriction endonuclease, recognizing and cleaving of nucleotide sequence between 5'-ATGCA' and T-3' ends.

EFFECT: simplified technology.

3 ex, 3 dwg

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RU 2 302 459 C2

Authors

Puchkova Larisa Ivanovna

Totmenina Ol'Ga Dmitrievna

Afonina Veronika Sergeevna

Polushkina Alla Fridrikhovna

Repin Vladimir Evgen'Evich

Dates

2007-07-10—Published

2005-09-22—Filed