METHOD OF COLLAGENASE PREPARING Russian patent published in 1998 - IPC

FIELD: biotechnology, biochemistry. SUBSTANCE: crab hepatopancreas tissue is homogenized in buffer solution, inert substances are extracted from homogenate and collagenase is isolated from solution and lyophilized. Inert substance extraction is carried out by addition of 0.2-1.5% chitosan solution in 1% acetic acid to homogenate as measured 0.2-1.4 l chitosan solution/1 kg crab hepatopancreas. Then mixture is stirred, settled for 1-3 h and filtered. Collagenase is isolated by ultrafiltration of enzyme solution on hollow fibers. The end product can be used in medicine, cosmetic, perfume and food industry and in leather-fur manufacture. EFFECT: improved method of preparing. 4 cl