METHOD OF PHYTOECDYSONE PREPARING Russian patent published in 1998 - IPC

FIELD: pharmacy, pharmacology. SUBSTANCE: invention relates to a method of preparing phytoecdysones used for express-analysis or raw and drugs. Milled rhizomes and roots of medicinal plant, Leuzea carthamoides, (size is less 0.25 mm) are extracted one time with 70% ethanol at constant stirring for 4 h at the room temperature at total ratio raw to extract 1:10. Extract is poured off and filtered. Extract is dried and treated successively firstly with 4 portions of ethyl acetate at total ratio of extract and extractant 1: 10 up to negative reaction with 1% solution of ferric (+3) chloride, then with 3 portions of 95% ethanol at total ratio of extract and extractant 1: 7 in the presence of aluminium oxide (the 2-d degree of activity by Brockman) at total ratio of extract and sorbent 1:3.5. Alcoholic extracts obtained by three-fold extraction are combined and used for quantitative analysis of phytoecdysone sum. EFFECT: simplified method, decreased cost, increased yield of product.