FIELD: biotechnology.
SUBSTANCE: invention relates to molecular diagnostics. Described is a method for identifying a phytopathogenic fungus Fusarium oxysporum by PCR, based on the use of species-specific primer pairs. Method comprises recovering DNA from samples of biological material, carrying out a polymerase chain reaction with the recovered DNA and species-specific primers, electrophoresis of PCR products in agarose gel, their visualization and identification by size comparison with DNA fragments of a molecular size marker. Presence of 285 base pairs of PCR products in sample or 370 base pairs indicates the presence of Fusarium oxysporumin the analysed sample.
EFFECT: wider range of means for identifying DNA of phytopathogenic fungi Fusarium oxysporum in biological material.
1 cl, 1 tbl, 2 ex
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