FIELD: molecular diagnostics.
SUBSTANCE: invention concerns the detection and identification of DNA of the phytopathogenic fungi Alternaria solani Sorauer in biological material using polymerase chain reaction (PCR). A method for identifying the phytopathogenic fungus Alternaria solani by PCR is proposed, based on the use of species-specific primer pairs for PCR AltS1-F: 5' - AAG GAC САА ССС ATA AAC СТТ ТТТ GCA ATG-3' / AltS1-R: 5' - GAC ТТТ AAG GCG AGT CTC CCG CAA G-3', or AltS2-F: 5' - CTC GTC CGG CCG GAC СТТ CCT TTT AGC-3' / AltS2-R: 5' - TCT CGT AAG GTG CCG AGC GAG TCA GA-3'. The method involves isolating DNA from samples of biological material, carrying out a polymerase chain reaction with isolated DNA and species-specific primers, electrophoresis of PCR products in an agarose gel, their visualization and identification by comparison in size with marker DNA fragments of a molecular weight marker and/or amplicons of a control sample. The presence of PCR products of 325 or 463 nucleotide pairs in size when using primers AltS1-F and AltS1-R or AltS2-F and AltS2-R, respectively, in the sample indicates the presence of Alternaria solani in the sample under study.
EFFECT: method allows for early diagnosis of Alternaria blight on nightshade plants in the field, as well as carrying out phytosanitary control of the presence of this phytopathogen.
1 cl, 1 tbl, 2 ex
Title | Year | Author | Number |
---|---|---|---|
METHOD FOR PCR IDENTIFICATION OF PHYTOPATHOGENIC FUNGUS FUSARIUM OXYSPORUM | 2022 |
|
RU2816852C1 |
METHOD FOR ANALYSING DIFFERENTIALLY METHYLATED GENOMIC REGIONS IN BIOLOGICAL SAMPLES OF BONE MARROW AND BLOOD OF CHILDREN WITH ACUTE MYELOID LEUKAEMIA | 2017 |
|
RU2689727C2 |
METHOD FOR QUANTITATIVE DETERMINATION OF SPECIES COMPOSITION OF PROPIONIBACTERIA DWELLING ON HUMAN SKIN | 2013 |
|
RU2542477C2 |
METHOD FOR OBTAINING A LINE OF HUMANIZED MICE CONTAINING 3974insT INSERTION IN THE mGrin2a GENE (MICE GLUTAMATE [NMDA] RECEPTOR SUBUNIT EPSILON-1), LEADING TO PREMATURE TERMINATION OF TRANSLATION OF THE grin2a PROTEIN | 2021 |
|
RU2764650C1 |
METHOD OF OBTAINIG AN ATTENUATED PLASMID-FREE STRAIN OF F.TULARENSIS 15 CMSA, SYNTHESIZING MYCOBACTERIAL ANTIGEN SUPEROXIDE DISMUTASE A | 2019 |
|
RU2745161C1 |
METHOD FOR IDENTIFICATION OF SOYA BREEDS ON BASIS OF MICROSATELLITE (SSR) MARKERS | 2008 |
|
RU2388828C1 |
SEQUENCE OF DNA-APTAMERS, BINDING TO PROTEOLYTIC SUBUNIT OF NEUROTOXIN OF TYPE A CLOSTRIDIUM BOTULINUM | 2014 |
|
RU2571210C1 |
GENETIC CONSTRUCT ADAPTED TO DELIVER THE HUMAN SMN1 GENE WITH ADENO-ASSOCIATED VIRUS OF SEROTYPE 2 TO PROVIDE NEUROSPECIFIC EXPRESSION | 2022 |
|
RU2801848C1 |
METHOD FOR EARLY PCR DIAGNOSTICS OF THE ALEUTIAN MINK DISEASE VIRUS CARNIVORE AMDOPARVOVIRUS | 2020 |
|
RU2756924C2 |
METHOD FOR DETECTION OF GENOTYPES 1 AND 2 OF EPSTEIN-BARR VIRUS | 2022 |
|
RU2789353C1 |
Authors
Dates
2023-10-03—Published
2022-12-30—Filed