FIELD: virology, molecular biology, genetic engineering. SUBSTANCE: method involves construction of recombinant plasmid pFS 19 combining gene determining resistance to formaldehyde FOR-R as a dominant selective marker and gene S encoding the surface antigen of virus hepatitis B expression of that is regulated by hybrid promoter GAL 1-10-PYKI and transcription terminator PGK1 with DNA fragment 2-mcm of yeast plasmid containing ori and with gene that determines resistance to ampicillin with E. coli ori. At transformation of yeast Saccharomyces cerevisiae with obtained plasmid expression level of the surface antigen is 50-160 mg/1 cultural fluid. Invention provides preparing the surface antigen of virus hepatitis B in yeast S. cerevisiae. EFFECT: increased yield of antigen. 2 tbl, 7 dwg
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Year |
Author |
Number |
| RECOMBINANT PLASMID DNA PHBS PROVIDING BIOSYNTHESIS OF SURFACE ANTIGEN OF HEPATITIS B VIRUS, METHOD OF CONSTRUCTION OF PLASMID, YEAST STRAIN PICHIA PASTORIS PS103 (PHBS) AS PRODUCER OF INDICATED ANTIGEN |
2000 |
- Padkina M.V.
- Parfenova L.V.
- Sambuk E.V.
- Smirnov M.N.
|
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| RECOMBINANT PLASMID ENCODING HEPATITIS B VIRUS SURFACE ANTIGEN (HBSAG), ITS PREPARING AND YEAST STRAIN HANSENULA POLYMORPHA AS PRODUCER OF HEPATITIS B VIRUS SURFACE ANTIGEN (HBSAG) |
2002 |
- Lunin V.G.
- Timofeeva T.V.
- Suchkov A.V.
|
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| RECOMBINANT PLASMID DNA PDES 20 ENCODING THE SURFACE ANTIGEN OF HEPATITIS B VIRUS (HBSAG/AYW), THE YEAST STRAIN SACCHAROMYCES CEREVISIAE CONTAINING THE RECOMBINANT PLASMID DNA PDES 20 - A PRODUCER OF THE SURFACE ANTIGEN OF HEPATITIS B VIRUS (HBSAG/AYW) |
1996 |
- Drutsa V.L.
- Budanov M.V.
- Borisova V.N.
- Jakovleva I.M.
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| RECOMBINANT PLASMID DNA DETERMINING SYNTHESIS OF HUMAN FIBROBLAST INTERFERON IN YEAST CELLS, METHOD OF ITS CONSTRUCTION AND YEAST STRAIN SACCHAROMYCES CEREVISIAE AS PRODUCER OF HUMAN FIBROBLAST INTERFERON |
1998 |
- Padkina M.V.
- Parfenova L.V.
- Sambuk E.V.
- Smirnov M.N.
|
RU2180003C2 |
| STRAIN OF YEAST SACCHAROMYCES CEREVISIAE CONTAINING RECOMBINANT PLASMID YEP 63/AB, - A PRODUCER OF HUMAN HEPATITIS B VIRUS M-PROTEIN DERIVATIVE |
1994 |
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- Ehl'Darov Mikhail Anatol'Evich[Ru]
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RU2082759C1 |
| RECOMBINANT PLASMIS DNA pZEN16 FOR TRANSFER AND EXPRESSION OF GENES IN MYCELIAL FUNGUS ACREMONIUM CHRYSOGENUM |
2009 |
- Zhgun Aleksandr Aleksandrovich
- Noskov Viktor Vladimirovich
- Kerpichnikov Ivan Viktorovich
- Ehl'Darov Mikhail Anatol'Evich
- Dumina Marija Vladimirovna
|
RU2434944C2 |
| STRAIN OF YEAST PICHIA PASTORIS AS PRODUCER OF LEUKOCYTE HUMAN INTERFERON-16, RECOMBINANT PLASMID DNA PHIN AND METHOD FOR ITS CONSTRUCTION |
2002 |
- Padkina M.V.
- Parfenova L.V.
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|
RU2203950C1 |
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1999 |
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- Boldyreva E.F.
- Shingarova L.N.
- Miroshnikov A.I.
- Gavrikov V.G.
- Stepanov A.V.
- Kalinin Ju.T.
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RU2143493C1 |
| YEAST STRAIN PICHIA PASTORIS AS PRODUCER OF HUMAN FIBROBLAST INTERFERON, RECOMBINANT PLASMID DNA PHIS AND METHOD OF ITS CONSTRUCTION |
2000 |
- Padkina M.V.
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| RECOMBINANT PLASMID DNA PJ DB (MSIL), ENCODING OF HUMAN INTERLEUKIN-2 SYNTHESIS IN YEAST CELLS SACCHAROMYCES CEREVISIAE, METHOD OF ITS PREPARATION, AND YEAST STRAIN SACCHAROMYCES CEREVISIAE - A PRODUCER OF HUMAN INTERLEUKIN-2 |
0 |
- Myasnikov Andrej Novomirovich
- Smirnov Mikhail Nikolaevich
- Avot Andris Yanovich
- Gren Elmar Yanovich
- Romanchikova Nadezhda Viktorovna
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|
SU1770359A1 |
