METHOD OF PURIFICATION OF HEPATITIS B SURFACE ANTIGEN CONTAINING PRE-S2-PEPTIDE FROM RECOMBINANT YEAST CELLS, VACCINE FOR IMMUNIZATION AGAINST HEPATITIS B Russian patent published in 1998 - IPC

Abstract RU 2122430 C1

FIELD: medicine, virology. SUBSTANCE: recombinant yeast cells expressing a surface antigen are disrupted in buffer containing chaotropic salt and a surface-active substance. Extract is alkalinized to pH 11.0-13.5 and then acidified to pH 4.5-6.0. The further antigen purification is carried out by adsorption on silicon anhydride, desorption with urea-containing buffer and gel-filtration. Method ensures to decrease an antigen destruction with proteases. Vaccine containing pre-S2-peptide is active with respect to different variants of hepatitis B virus. Method can be used in biotechnology and immunology. EFFECT: improved method of purification. 15 cl, 1 dwg, 2 tbl, 5 ex

Similar patents RU2122430C1

Title	Year	Author	Number
METHOD FOR PREPARING HEPATITIS B VIRUS SURFACE ANTIGEN FROM RECOMBINANT YEAST CELLS HANSENULA POLYMORPHA AND VACCINE FOR IMMUNIZATION AGAINST HEPATITIS B	2002	Lebedin Ju.S. Timofeeva T.V. Suchkov A.V.	RU2205023C1
STRAIN OF HUMAN EMBRYO LUNG FIBROBLAST DIPLOID CELLS USED FOR VIRUS CULTURING	1997	Jeong Min Kim Bok Rajon Park Dzho Khong Park	RU2146289C1
METHOD OF PREPARING SURFACE ANTIGEN OF HEPATITIS B VIRUS (HBS AG), MONOCLONAL ANTIBODIES CB-HEPI, SURFACE ANTIGEN OF HEPATITIS B VIRUS (HBS AG) AND METHOD OF PREPARING HYBRID CLONE 48/1/574 PRODUCING MONOCLONAL ANTIBODIES CB-HEPI	1992	Verena Lusila Musio Gonsales Ehduardo Penton Arias Kh'Juvel' Fontirrochi Ehskobar Marselo Nasabal' Gal'Ves Marta De Gonsales Gr'Ego Alekhandro Beldarrain Isnaga Gil'Ermo Padron Gonsales Viktoria Ramires Al'Bakhes Arnal'Do Garsia Pen'Ja Karlos Ehnrike Ruis Krus Gladis Mabel' Isk'Erdo Lopes Luis Ehrrera Martines Khose Garsia Suares Gustavo De Lja Riva De Lja Riva Ramon Aleksis Sant'Jago Avila Karlos Duarte Kano Lilia Peres Suares	RU2128707C1
TRANSFORMED STRAIN OF YEAST HANSENULA POLYMORPHA AS PRODUCER OF RECOMBINANT SURFACE ANTIGEN OF HEPATITIS VIRUS B-HBSAG/ADW	2003	Borisova V.N. Budanov M.V. Jakovleva I.M. Mel'Nikov V.A.	RU2235768C1
METHOD OF PREPARING HEPATITIS B SURFACE ANTIGEN PURIFIED PARTICLES, PURIFIED PARTICLE OF HUMAN HEPATITIS B SURFACE ANTIGEN AND VACCINE AGAINST HEPATITIS B	1990	Ziv Iven-Chen[Il]	RU2080876C1
RECOMBINANT VACCINE FOR PREVENTING VIRAL HEPATITIS B (VARIANTS)	2003	Borisova V.N. Budanov M.V. Jakovleva I.M. Mel'Nikov V.A.	RU2238105C1
TRANSFORMED YEAST STRAIN PICHIA ANGUSTA AS PRODUCER OF RECOMBINANT SURFACE ANTIGEN HBSAG/AYW OF HEPATITIS B VIRUS	2003	Borisova V.N. Budanov M.V. Jakovleva I.M. Mel'Nikov V.A. Agafonov M.O. Ter-Avanesjan M.D.	RU2230782C1
PREPARATION OF VACCINES CONTAINING HEPATITIS TYPE B VIRUS SURFACE ANTIGEN AND SURFACE-ACTIVE MATERIAL	2006	Kontorni Mario	RU2444374C2
MONOCLONAL ANTIBODY BINDING WITH HEPATITIS B VIRUS SURFACE ANTIGEN, FAB-FRAGMENT AND METHOD OF DECREASE OF LEVEL OF CIRCULATING SURFACE ANTIGEN OF HEPATITIS B VIRUS ANTIGEN IN PATIENTS	1992	Lars G.Ostberg	RU2146706C1
POLYEPITOPIC ANTI-HEPATITIS B VACCINE OF 4-TH GENERATION AND METHOD OF OBTAINING IT	2011	Granovskij Nikolaj Nikolaevich Granovskij Vladimir Nikolaevich	RU2469741C1

RU 2 122 430 C1

Authors

Sun-Dzhae Park

Jang-Mi Li

Kiung-Khi Jun

Kuk-Dzhin Lim

Jang-San Kvon

Dates

1998-11-27—Published

1995-12-08—Filed