COVALENTLY BOUND COMPLEXES OF OLIGONUCLEOTIDE-NUCLEIC ACID, METHOD OF THEIR SYNTHESIS Russian patent published in 1999 - IPC

FIELD: bioorganic chemistry. SUBSTANCE: invention relates to covalently bound complexes of oligonucleotide-nucleic acid of the general formula (I) and (II) where R - oligodeoxyribonucleotide residue; R′,R″,R″′ - nucleic acid residues; n = 3-12. Compounds of the formulas (I) and (II) are synthesized by reaction of nucleic acid and an active oligonucleotide probe taken at equimolar ratio in an aqueous buffer solution under conditions of stability of complementary complex at pH 6.0-9.0. The use of oligodeoxyribonucleotides of the formulas (III): RO-X-Y, (IV): Y-X-OR and (V): Y-X-ORO-X-Y where X is as active oligonucleotide probes ensures to eliminate an additional stage of addition of reactive group and increase selectivity of complexes (I) and (II) formation. EFFECT: improved method of synthesis. 2 cl, 4 dwg, 1 tbl, 12 ex