PROCESS FOR PREPARING HYALURONIDASE Russian patent published in 1999 - IPC

Abstract RU 2126044 C1

FIELD: preparation of gualuronidase enzyme from milt. SUBSTANCE: disintegrated milt is subjected to acid extraction. Extract is centrifuged and separated. Clarified extract is filtered on nutsch filter and directed to column with macroporous cationite. Enzyme is eluted from carrier at pH lower or equal to 9.0 since at pH of higher than 9.0 gel formed makes it difficult for solution to flow. Desalting with ammonium sulfate is carried out at saturation degree of 0.82-0.85 to recover monomeric forms of hyalurodinase. EFFECT: more efficient preparation process. 3 cl, 1 ex, 1 tbl

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RU 2 126 044 C1

Authors

Glazova Natalija Vladimirovna

El'Kin Gelij Ehmil'Evich

Zainkova Natalija Vacheslavovna

Igonina Ljudmila Mikhajlovna

Popova Julija Mikhajlovna

Rudometova Natalija Viktorovna

Junkerova Ljudmila Ivanovna

Dates

1999-02-10Published

1995-02-23Filed