METHOD OF ENZYME PREPARATION PREPARING Russian patent published in 1999 - IPC

Abstract RU 2133771 C1

FIELD: biotechnology, biochemistry, microbiology. SUBSTANCE: biomass obtained in microorganism-producer culturing is extracted in an ultrasonic oscillation field and purified by microfiltration. Extract is concentrated by ultrafiltration, enzymes are precipitated with alcohol or acetone. Enzymes are dried by precipitate washing out with liquid carbon dioxide under pressure above atmospheric one and the following pressure drop to an atmospheric value. Ultrasonic oscillations are generated at condensation in an extraction mixture of extractant vapors bubbling in its. Invention can be used for production of enzyme preparations from microbiological raw. EFFECT: intensified process, accelerated extraction, simplified precipitate drying, decreased consumption of reagents and energy.

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RU 2 133 771 C1

Authors

Kvasenkov O.I.

Jur'Ev D.N.

Egorov A.D.

Ratnikov A.Ju.

Andreev V.G.

Dates

1999-07-27Published

1997-02-17Filed