FIELD: biotechnology, biochemistry, microbiology. SUBSTANCE: biomass obtained in microorganism-producer culturing is extracted in an ultrasonic oscillation field and purified by microfiltration. Extract is concentrated by ultrafiltration, enzymes are precipitated with alcohol or acetone. Enzymes are dried by precipitate washing out with liquid carbon dioxide under pressure above atmospheric one and the following pressure drop to an atmospheric value. Ultrasonic oscillations are generated at condensation in an extraction mixture of extractant vapors bubbling in its. Invention can be used for production of enzyme preparations from microbiological raw. EFFECT: intensified process, accelerated extraction, simplified precipitate drying, decreased consumption of reagents and energy.
Title | Year | Author | Number |
---|---|---|---|
METHOD OF PRODUCTION OF ENZYME PREPARATIONS | 1997 |
|
RU2126448C1 |
METHOD OF PRODUCING MILK-CLOTTING ENZYME FROM BIOMASS OF FRUIT BODIES OF HIGHER FUNGI | 2022 |
|
RU2815049C1 |
METHOD FOR PRODUCING DRY SOLUBLE CHICORY EXTRACT | 2020 |
|
RU2760434C1 |
STRAIN BACILLUS LICHENIFORMIS BST-1 AS PRODUCER OF PENICILLINASE AND METHOD FOR PREPARING PENICILLINASE | 2002 |
|
RU2221041C1 |
METHOD FOR MAKING ENZYME PREPARATION OF FISH MATERIALS | 2007 |
|
RU2352634C1 |
METHOD OF PREPARING FOOD PROTEIN FROM VEGETABLE RAW | 1998 |
|
RU2124844C1 |
METHOD OF GLYCEROL OXIDASE PREPARING | 1995 |
|
RU2117702C1 |
PLANT BEVERAGE | 2003 |
|
RU2265383C2 |
METHOD OF AGENT MAKING FOR PLANT TREATMENT | 1996 |
|
RU2075934C1 |
HYALURONIDASE PREPARATION AND METHOD OF ITS PREPARATION | 2016 |
|
RU2671537C2 |
Authors
Dates
1999-07-27—Published
1997-02-17—Filed