FIELD: medicine.
SUBSTANCE: there is designed recombinant plasmid DNA pSC13D6 containing a gene of one-chained antibody to tick-borne encephalitis virus sc13D6. Plasmid consists of 3782 basepairs and contains: a plasmid vector pGEMl, phage T7 promotor and unique restriction sites. There is also discovered strain of bacteria Escherichia coli BL21 (DE3)/pSC13D6 - a producer of a virus neutralising one-chained antibody sc13D6 to tick-borne encephalitis virus.
EFFECT: invention allows producing one-chained antibodies sc13D6 to tick-borne encephalitis virus able to inhibit it effectively.
2 cl, 5 dwg, 5 ex
| Title | Year | Author | Number |
|---|---|---|---|
| RECOMBINANT PLASMID DNA PTLS CONTAINING GENE OF SINGLE-STRANDED ANTIBODY AGAINST TICK-BORNE ENCEPHALITIS VIRUS AND STRAIN OF BACTERIUM ESCHERICHIA COLI AS PRODUCER OF SINGLE-STRANDED ANTIBODIES AGAINST TICK-BORNE ENCEPHALITIS VIRUS | 1998 |
|
RU2158309C2 |
| RECOMBINANT PLASMID DNA P7SHSHIS FOR PRODUCTION OF DIMERIC SINGLE-STRANDED ANTIBODY AGAINST TICK-BORNE ENCEPHALITIS VIRUS AND STRAIN OF ESCHERICHIA COLI AS PRODUCER OF DIMERIC FORM OF SINGLE-STRANDED ANTIBODY AGAINST TICK-BORNE ENCEPHALITIS VIRUS | 2000 |
|
RU2190017C2 |
| RECOMBINANT PLASMID pHis6-flagG-protE, WHICH PROVIDES SYNTHESIS OF A RECOMBINANT CHIMERIC PROTEIN, INCLUDING EPITOPES OF GLYCOPROTEIN E OF TICK-BORNE ENCEPHALITIS VIRUS AND FLAGELLIN G SALMONELLA TYPHII AND USED AS A BASE FOR VACCINE AGAINST TICK-BORNE ENCEPHALITIS VIRUS | 2018 |
|
RU2702716C2 |
| RECOMBINANT DNA PLASMID PGSDE1 ENCODING TICK-BORNE ENCEPHALITIS VIRUS PROTEIN E AND STRAIN OF ESCHERICHIA COLI - PRODUCER OF TICK-BORNE ENCEPHALITIS VIRUS PROTEIN E | 1997 |
|
RU2136754C1 |
| RECOMBINANT PLASMID DNA pCLm4/hygro-14D5 CODING POLYPEPTIDE WITH LIGHT CHAIN PROPERTIES OF CHIMERIC ANTIBODY TO TICK-BORNE ENCEPHALITIS VIRUS, AND RECOMBINANT PLASMID DNA pCHm2-14D5 CODING POLYPEPTIDE WITH HEAVY CHAIN PROPERTIES OF CHIMERIC ANTIBODY TO TICK-BORNE ENCEPHALITIS VIRUS, CHIMERIC ANTIBODY PROVIDING ACUTE PREVENTION OF TICK-BORNE ENCEPHALITIS IN MICE | 2013 |
|
RU2550252C1 |
| RECOMBINANT PLASMID DNA PFLAG -SC14D5A-NLUC-R1 AND PFLAG-NLUC-R2-SC14D5A, PROVIDING SYNTHESIS OF HYBRID PROTEINS SC14D5A-NLUC-R1 AND NLUC-R2-SC14D5A, AND HYBRID PROTEINS SC14D5A-NLUC-R1 AND NLUC-R2-SC14D5A, BINDING PROTEIN E OF TICK-BORNE ENCEPHALITIS VIRUS WITH COMPLEMENTATION OF NLUC FRAGMENTS-R1 AND NLUC-R2 AND WITH THE BIOLUMINESCENT ACTIVITY | 2022 |
|
RU2784817C1 |
| RECOMBINANT PLASMID DNA pFLAG-sc14D5a-Rm7 ENSURING SYNTHESIS OF HYBRID PROTEIN sc14D5a-Rm7, BACTERIA STRAIN Escherichia coli - PRODUCER OF HYBRID PROTEIN sc14D5a-Rm7, AND HYBRID PROTEIN sc14D5a-Rm7, COMBINING PROTEIN E OF TICK-BORNE ENCEPHALITIS MICE AND HAVING BIO-LUMINESCENCE ACTIVITY | 2014 |
|
RU2565545C1 |
| mRNA-BASED IMMUNOBIOLOGICAL AGENT FOR PREVENTION OF DISEASES CAUSED BY TICK-BORNE ENCEPHALITIS VIRUS | 2024 |
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RU2823754C1 |
| RECOMBINANT PLASMID DNA PC-NS3 PROVIDING INTEGRATION OF COMPLEX OF GENES C, PRM, E, NS1, NS 2A, NS 2B, NS3 OF TICK-BORNE ENCEPHALITIS VIRUS IN GENOME OF BOVINE VACCINE VIRUS AND RECOMBINANT STRAIN OF BOVINE VACCINE VIRUS EXPRESSING COMPLEX OF GENES C, PRM, E, NS1, NS 2A, NS2B, NS3 OF TICK-BORNE ENCEPHALITIS VIRUS IN IMMUNIZED ORGANISM CELLS | 1995 |
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| IMMUNOBIOLOGICAL AGENT FOR THE PREVENTION OF DISEASES CAUSED BY TICK-BORNE ENCEPHALITIS VIRUS BASED ON A RECOMBINANT VIRUS OF THE GENUS FLAVIVIRUS | 2022 |
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RU2795800C1 |
