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2 144 083

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IPC

(21) (22)

Application

98105296/13, 1998-03-13

(24)

Start date

1998-03-13

(22)

Actual filing date

1998-03-13

(45)

Published

2000-01-10

(72)

Inventor

Naumova N.V.Pikalova M.I.Domanova Z.M.Khajbullina I.I.Gileva I.P.Kolokol'Tsov A.A.

(73)

Holder

Gosudarstvennyj Nauchnyj Tsentr Virusologii I Biotekhnologii

METHOD OF INDUSTRIAL PRODUCING HUMAN RECOMBINANT GRANULOCYTE-MACROPHAGE COLONY-STIMULATING FACTOR Russian patent published in 2000 - IPC

Abstract RU 2144083 C1

FIELD: biotechnology. SUBSTANCE: recombinant strain of Escherichia coli Sg20050/p280 gM is cultured on standard LB-medium at 25-37 C. Culturing is carried out in the presence of ampicillin and tetracycline. The concentration of dissolved oxygen is 10-40% of saturation. Culturing can be performed at 29 ± 1 C. Culturing can be performed for three stages at successive increase of nutrient medium volume from 50 ml to 100 l. Invention ensures to provide the maximal accumulation of glycoprotein of granulocyte- -macrophage colony-stimulatin factor in bacterium cells. EFFECT: improved method of the factor producing, increased yield of the end product. 3 cl, 3 tbl, 2 ex

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RU 2 144 083 C1

Authors

Naumova N.V.

Pikalova M.I.

Domanova Z.M.

Khajbullina I.I.

Gileva I.P.

Kolokol'Tsov A.A.

Dates

2000-01-10—Published

1998-03-13—Filed