FIELD: medicine, microbiology, vaccine prophylaxis. SUBSTANCE: method involves addition of 0.1 mcg of synthetic polyelectrolyte - polyoxydonium dissolved in phosphate buffer to 0.1 mcg of protective antigen (by protein level) followed by stirring on magnetic mixer at 37 C for 20 min and then at 3-6 C for 3 h and filtration through filter (pore diameter is 0.45 mcm). Invention can be used in construction of areactogenic whooping cough vaccine preparation. Invention ensures to obtain conjugated protective whooping cough antigen based on synthetic carrier - polyoxydonium exhibiting high immunogenic properties (95% of viability). EFFECT: improved method of preparing, high quality and properties of antigen. 1 tbl
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