METHOD FOR CARRYING OUT IN VITRO FERTILIZATION Russian patent published in 2000 - IPC

FIELD: medicine. SUBSTANCE: method involves making oocyte puncture and introducing one or several such oocytes into container filled with culturing medium without air or carbon dioxide being introduced. The medium provides conditions for fertilizing and culturing oocytes and spermatozoa. Closed container is introduced into vaginal cavity or uterus to fertilize oocyte(s) and culture the embryo(s). The process is conducted without cycle stimulation being applied under LH control by determining its contents in blood and programming oocyte puncture depending on LH value. Before being introduced into vaginal cavity, the container is optionally hold at 37 C temperature for 2-6 h to reach mature state. Equipment set has device for determining LH contents in express mode, needle for making oocyte puncture, filtering unit for preparing sperm, container for fertilizing and/or maturing oocyte(s) and culturing embryos and culturing media. EFFECT: simplified fertilization procedure; high quality of fertilization. 17 cl