METHOD FOR PREPARING SAMPLES FOR DIAGNOSING THE CASES OF PSEUDOTUBERCULOSIS USING POLYMERASE CHAIN REACTION Russian patent published in 2000 - IPC

Abstract RU 2153671 C1

FIELD: medicine. SUBSTANCE: method involves diluting raw material (feces) with buffered physiological salt solution 1.10, treating it with 0.72% potassium hydroxide (1:1), adding buffer solution (pH 7.2-7.4) for neutralizing pH in 1:2 proportion, centrifuging during 2-5 min under 10000 rev/min. The precipitate is washed twice with distilled water centrifuging it every time, and solved in 20 mcl of deionized water. The solved precipitate is heated at 100 C during 10 min. The sample is usable in setting polymerase chain reaction. EFFECT: accelerated diagnosis method.

Similar patents RU2153671C1

Title Year Author Number
METHOD OF ISOLATION OF DNA FROM MICROORGANISMS AND ANIMAL CELLS SUITED FOR POLYMERASE CHAIN REACTION CARRYING OUT 1997
  • Shestopalov M.Ju.
  • Balakhonov S.V.
  • Kalinovskij A.I.
  • Golubinskij E.P.
RU2129610C1
STRAIN OF HYBRID CULTURED MAMMALIAN CELLS MUS MUSCULUS L - A PRODUCER OF MONOCLONAL ANTIBODIES SHOWING SPECIFICITY TO BRUCELLA PROTEIN PREPARATION OF MOLECULAR MASS 18 AND 38 KDA 1996
  • Mikhajlov L.M.
  • Titenko A.M.
  • Zakhlebnaja O.D.
  • Laukner I.V.
RU2113475C1
METHOD OF ASSAY OF BACTERIAL LIPOPOLYSACCHARIDES 1996
  • Chernov A.B.
  • Markov E.Ju.
RU2114436C1
METHOD FOR PREPARING DIAGNOSTIC KIT FOR DETECTION OF CORPUSCULAR AND SOLUBLE BRUCELLA ANTIGENS DUE TO DOT-IMMUNOASSAY 2000
  • Zagoskina T.Ju.
  • Golubinskij E.P.
  • Kalinovskij A.I.
  • Dokorina A.A.
RU2202800C2
METHOD FOR DETECTING BRUCELLA ANTIGENS 2000
  • Zagoskina T.Ju.
  • Golubinskij E.P.
  • Kalinovskij A.I.
  • Markov E.Ju.
  • Dokorina A.A.
RU2202799C2
METHOD OF DETECTION OF PIG CLASSIC PLAGUE VIRUS 1997
  • Semenikhin V.I.
  • Puzyrev A.T.
  • Donchenko A.S.
  • Oreshkova S.F.
  • Chekishev V.M.
  • Il'Ichev A.A.
  • Gerb P.E.
RU2120994C1
METHOD FOR PREPARING DIAGNOSTIC KIT FOR DETECTION OF ANTIBRUCELLOSIS ANTIBODIES IN BLOOD SERUM DUE TO DOT-IMMUNOASSAY 2000
  • Zagoskina T.Ju.
  • Kalinovskij A.I.
  • Golubinskij E.P.
  • Dokorina A.A.
RU2202798C2
STRAIN OF BACTERIUM BRUCELLA MELITENSIS BIOVARA I USED FOR PREPARING OF POLYVALENT HYPERIMMUNE SERUM TO BRUCELLAS 1991
  • Laukner I.V.
  • Zakhlebnaja O.D.
RU2005781C1
STRAIN OF BACTERIUM VIBRIO CHOLERAE 0139 SEROVARA KM 115 (I-1262), AVIRULENT TEST-STRAIN NO CONTAINING OPERON CTX AB, FOR IMMUNOLOGICAL AND GENETIC INVESTIGATIONS 1999
  • Ganin V.S.
  • Urbanovich L.Ja.
  • Maramovich A.S.
  • Balakhonov S.V.
  • Mel'Nikova Z.V.
RU2149898C1
METHOD FOR DETECTING ANTIGENS OF TULAREMIAL MICROBE 2001
  • Nikolaev V.B.
  • Zagoskina T.Ju.
  • Markov E.Ju.
  • Dokorina A.A.
  • Mironchuk Ju.V.
  • Gefan N.G.
  • Golubinskij E.P.
RU2203496C2

RU 2 153 671 C1

Authors

Klimov V.T.

Breneva N.V.

Chesnokova M.V.

Maramovich A.S.

Dates

2000-07-27Published

1999-09-07Filed