METHOD OF ISOLATION OF DNA FROM MICROORGANISMS AND ANIMAL CELLS SUITED FOR POLYMERASE CHAIN REACTION CARRYING OUT Russian patent published in 1999 - IPC

FIELD: biotechnology, genetic engineering, biochemistry. SUBSTANCE: for isolation of DNA an analyzed sample in volume 5-10 mcl is placed to 182 mcl lysing-precipitating solution consisting of 38 mcl lysing solution: 3.45 M NaOH, 0.36 M NaCl and 144 mcl 96% ethanol, stirred and kept at the room temperature for 10-15 min. Then mixture is centrifuged at 12000-14000 rev/min for 5 min, supernatant is discarded, sediment is washed out with 500 mcl 80% ethanol once followed by centrifugation at 12000-14000 rev/min for 2 min. Supernatant is discarded and sediment is dried at the room temperature for 5-10 min. To dry precipitate 15-30 mcl TE-buffer is added and stirred. Effectiveness of DNA isolation is based on lysis of cellular wall resistant to effect of the most of lysing agents. EFFECT: decreased cost and laboriousness of method, simplified procedure, ecological safety due to excluding toxic chemical reagents using. 2 ex