FIELD: medicine.
SUBSTANCE: invention refers to biotechnology and genetic engineering, namely to a genetically engineered construct pGoatcasGCSF for human granulocyte colony-stimulating factor (GCSF) expression . The offered invention can be used for producing transgenic animals that are human granulocyte colony-stimulating factor producers. It involves creating the genetically engineered construct pGoatcasGCSF of the size 6386 bps, and a specified nucleotide sequence shown in SEQ ID 1. The genetically engineered construct pGoatcasGCSF includes a 5'-regulatory sequence of the goat CSN1S1 asi-casein gene of the size 3387 bps connected with the full-size human GCSF gene of the size 1485 bps, and a 3'-flanking region of the cow gene CSN1S1 of the size 1514 bps.
EFFECT: stable effective level of human GCSF expression in milk of the transgenic animals, eliminated possibility of ectopic transgenic expression.
9 dwg, 2 tbl, 6 ex
| Title | Year | Author | Number |
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| GENETIC CONSTRUCTS LTF3, LTF5, LTF7, LTF10, LTF11 FOR PRODUCING RECOMBINANT HUMAN LACTOFERRIN (VERSIONS) | 2011 |
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| METHOD OF PRODUCING TRANSGENIC ANIMAL EXPRESSING HUMAN GRANULOCYTE COLONY-STIMULATING FACTOR IN MAMMARY GLAND AND HYBRID GENE H-GM-1 FOR METHOD REALIZATION | 1999 |
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| TRANSGENE FOR PREPARING THE RECOMBINANT POLYPEPTIDE IN TRANSGENIC COW MILK, A METHOD OF OBTAINING THE TRANSGENIC COW (VARIANTS), MILK FROM TRANSGENIC COW, FOOD COMPOSITION | 1990 |
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RU2806568C1 |
| RECOMBINANT STRAIN OF VV-GMCSF-S-Lact VACCINIA VIRUS, WITH ONCOLYTIC ACTIVITY, PRODUCING GRANULOCYTOR-MACROPHAGAL COLONY-STIMULATING HUMAN FACTOR AND SECRETED FORM OF LACTAPTIN ONCOTOXIC PROTEIN | 2016 |
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| DNA MOLECULE, EXPRESSION VECTOR | 1993 |
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