RECOMBINANT PLASMID DNA PKHBC-33 CONTAINING GENE OF HEPATITIS B VIRUS CORE ANTIGEN INVOLVING SEQUENCE OF THE MAIN NEUTRALIZING EPITOPE OF HEPATITIS B VIRUS SURFACE ANTIGEN (137-147) AND STRAIN OF BACTERIUM ESCHERICHIA COLI - A PRODUCER OF HEPATITIS B VIRUS CORE ANTIGEN WITH THE MAIN NEUTRALIZING EPITOPE OF HEPATITIS B VIRUS SURFACE ANTIGEN EXHIBITED ON ITS SURFACE Russian patent published in 1998 - IPC

Abstract RU 2112039 C1

FIELD: genetic and protein engineering, microbiological and medicinal industry. SUBSTANCE: invention relates to preparing recombinant plasmid DNA pKHBc-33 containing gene of hepatitis B virus core antigen (HBcAg) with the main neutralizing epitope of hepatitis B virus surface antigen (137-147) (HBsAg) exhibited on its surface and the corresponding strain-producer. Plasmid has also structural members that are necessary for hybrid protein expression providing in strain-producer cells. For preparing strain-producer competent cells of bacterium Escherichia coli JM103 are transformed with plasmid pKHBc-33. Obtained strain E. coli JM103/pKHBc-33 provides the induced synthesis of HBcAg-HBsAg (137-147) at expression level 5% of total cell protein. EFFECT: recombinant plasmid preparing. 2 cl, 3 dwg

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RU 2 112 039 C1

Authors

Karpenko L.I.

Chikaev N.A.

Melamed N.V.

Il'Ichev A.A.

Bajborodin S.I.

Dates

1998-05-27—Published

1995-10-24—Filed