METHOD OF MULTIPLE-ANALYTE IMMUNOASSAY Russian patent published in 2002 - IPC

FIELD: immunology, screening. SUBSTANCE: at the bottom of holes in microtitration plate one should immobilize the first biospecific components to different analytes under testing as discrete microareas, the total area of which is less or equal to that of a dry blood spot. Buffer : conjugate mixture with the second, either labeled or unlabeled, biospecific components to the certain analytes is applied right onto a dry blood spot. At the volume equivalent to its sorption capacity a dry blood spot is removed out of a hole. Reaction of biospecific binding with labeled Pt copro- or uroporphyrine by biospecific components is conducted in discrete microareas, holes are washed out, dried. Emission of label's phosphorescence is detected by subsequently scanning microareas by a focused laser ray. EFFECT: higher efficiency in providing improved sensitivity of an assay. 6 cl, 7 tbl, 16 dwg _