METHOD FOR SIMULTANEOUS DETECTION OF SEVERAL MARKERS OF CONGENITAL DISEASES IN DRY BLOOD STAINS Russian patent published in 2015 - IPC G01N33/577 

FIELD: medicine.

SUBSTANCE: invention refers to immunological methods of analysis and can be used for mass screening of congenital diseases in newborn children. That is ensured by immobilising in a microplate well first immune-specific components to thyrotropin, immunoreactive trypsin, thyroxin and 17α-OH-progesterone in the form of discrete microregions, placing in the well a paper disk of a dry blood sample, extracting the detectable markers from dry blood stains by adding into the well a buffer containing danazol and 8-anilinonaphthalene-1-sulfonic acid and 17α-OH-progesteron antibodies, which are bound to extracted 17α-OH-progesterone to form an immune complex with anti-species antibodies in the respective microregion simultaneously, then adding into the microplate well a reaction solution of second immune-specific components, containing a mixture of biotin-conjugated antibodies to thyrotropin, immunoreactive trypsin and thyroxin, and a 17α-OH-progesteron-protein-biotin conjugate for preparing the immune biotin-labelled complexes in the discrete microregions, removing the paper disk of the dry blood sample, adding a streptavidin and Pt-coproporphyrin conjugate to prepare the phosphorescent biotin-streptavidin complexes in the microregions on the bottom of the microplate well and detecting the label phosphoresce emission, by scanning the discrete microregions by a focused laser beam sequentially.

EFFECT: using the given method enables detecting biomarkers specifically and qualitatively in the newborn's capillary blood stain sample that enables diagnosing three newborn's congenital conditions.

10 cl, 7 dwg, 10 tbl