RECOMBINANT PLASMID DNA PPHO-SOD 23 ENCODING SYNTHESIS OF HUMAN SUPEROXIDE DISMUTASE AND STRAIN SACCHAROMYCES CEREVISIAE = D-11 VNIISCHM AS PRODUCER OF HUMAN SUPEROXIDE DISMUTASE Russian patent published in 2003 - IPC

FIELD: molecular biology, biotechnology, biochemistry, microbiological and medicinal industry. SUBSTANCE: invention relates to technology of preparing superoxide dismutase preparations (SOD). Recombinant plasmid DNA pPHO-SOD 23 encoding synthesis of human superoxide dismutase consisting of Eco RI/Bam HI-fragment p33 of expression cassette with gene SOD, Hind III/Bam HI-fragment with sequence of alcoholdehydrogenase transcription terminator and Bam HI-fragment with sequence of hepatitis B Hbs- antigen. The strain of yeast Saccharomyces cerevisiae = D-11 VNIISCHM as a producer of superoxide dismutase obtained by transformation of cells of recipient strain 20B12 Saccharomyces cerevisiae with genotype trPU with plasmid DNA pPHO-SOD23 exhibits the ability to selective maintaining expression plasmid in media of any composition no containing tryptophan. The strain allows to enhance the yield of SOD significantly (SOD content in lysate is 4 200 mcg/ml of lysate). EFFECT: enhanced yield of enzyme. 2 cl, 2 dwg, 1 ex