HIGH ACTIVITY MUTEINS OF INTERLEUKIN-4 Russian patent published in 2003 - IPC

FIELD: medicine. SUBSTANCE: human IL-4 has amino acid sequence corresponding to IL- 4 of wild type where mutein has one acid substitution in binding surface or alpha-spiral A or alpha-spiral C of wild type IL-4 by means of which mutein binds IL-4Rα receptor of at least greater affinity than the native IL-4. Substitution is preferentially selected a group of positions 13 and 16 in spiral A and positions 81 and 89 in spiral C. The most preferential embodiment is recombinant mutein of human IL- 4 where substitution in position 13 is Thr or Asp. Pharmaceutical compositions, amino acid and polynucleotide sequences coding muteins, transformed cell lines, antibodies against muteins and treatment methods are described. EFFECT: wide range of functional applications for supporting and recovering immune system. 37 cl, 6 dwg, 3 tbl