METHOD OF DIFFERENTIATION OF CORYNEBACTERIUM DIPHTHERIAE COMPRISING FUNCTIONALLY ACTIVE DIPHTHERIA TOXIN GENE FROM CORYNEBACTERIUM DIPHTHERIAE COMPRISING FUNCTIONALLY INACTIVE DIPHTHERIA TOXIN GENE Russian patent published in 2003 - IPC

FIELD: medicine, microbiology, biotechnology. SUBSTANCE: invention relates to method of differentiation of Corynebacterium diphtheriae comprising functionally active diphtheria toxin gene from Corynebacterium diphtheriae comprising functionally inactive diphtheria toxin gene. Indicated method involves isolation of DNA from the strain C. diphtheriae or from clinic material, carrying out polymerase chain reaction with simultaneous incorporation of primers 5'-GTCAAAGTGACGTATCCAGGA-3' and 5'-CACGGGTTTCAAAATTAATCTC-3', preparing amplicons of diphtheria toxin gene fragment (Sequence = 1), restriction of these amplicons with restriction endonuclease Bsm FI followed by electrophoresis of restriction fragments in agarose gel. Then differentiation is carried out by comparison of diphtheria toxin gene amplicons in analyzed sample with two control samples containing amplicons of functionally active and inactive diphtheria toxin gene. Invention provides acceleration and enhancement of precision of method. EFFECT: improved method of differentiation.