PURIFIED DNA POLYMERASE, RECOMBINANT DNA POLYMERASE, METHODS FOR THEIR PREPARING, ISOLATED DNA SEQUENCE AND SET FOR POLYMERASE CHAIN REACTION Russian patent published in 2004 - IPC

FIELD: biotechnology, molecular biology, biochemistry, enzymology. SUBSTANCE: invention relates to modified DNA polymerase. Invention claims the purified DNA polymerase eliciting activity of reverse transcriptase in the presence of magnesium ions and/or manganese ions and eliciting the reduced activity of 5'-3'-exonuclease and no eliciting activity of ribonuclease H and preparing from Carboxydothermus hydrogenoformans. Amino acid and nucleotide sequences are given in the description. Thermophilic DNA polymerase is a component of set using for carrying out polymerase chain reaction (PCR) with RNA reverse transcription. Invention provides preparing reverse transcriptase acting at high temperatures and providing producing DNA without deletions. EFFECT: improved preparing methods, valuable biological and biochemical properties of enzyme. 10 cl, 7 dwg, 3 ex