FIELD: biotechnology, biochemistry, microbiology. SUBSTANCE: DNA from microorganisms is isolated by disrupting bacterial cells with lysing solution containing 80% of phenol, 18.8% of H2O and 0.5% of NaOH (pH 9.3-9.5). Method provides preparing preparation of bacterial DNA with high purity and nativity degree. Invention can be used for isolation of DNA microorganisms. EFFECT: improved isolating method. 1 dwg, 1 ex
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Authors
Dates
2004-06-10—Published
2002-04-08—Filed