RECOMBINANT PLASMID DNA pPDGFB ENCODING A POLYPEPTIDE WITH HUMAN PLATELET-BB GROWTH FACTOR HUMAN PROPERTIES, AND A RECOMBINANT STRAIN OF METHYLOTROPHIC YEAST PICHIA PASTORIS, A PRODUCER OF A POLYPEPTIDE WITH HUMAN PLATELET-BB GROWTH FACTOR HUMAN PROPERTIES Russian patent published in 2018 - IPC C12N1/19 C12N15/81 C12N15/64 

FIELD: biotechnology.

SUBSTANCE: present invention relates to biotechnology and in particular to genetic engineering and can be used in medical studies to obtain a human platelet growth factor. Recombinant plasmid DNA pPDGFB encoding the synthesis of a polypeptide with human platelet-BB growth factor, which provides biosynthesis with a level of about 40÷60 mg of recombinant protein/liter culture. Highly efficient synthesis of the target polypeptide is ensured by the fact that the target gene is under the control of the strong inducible promoter AOX1.

EFFECT: present Pichia pastoris strain GS115PDGFB provides the synthesis of a polypeptide with human platelet-BB growth factor in an amount of at least 40 mg of the target protein per liter of culture.

2 cl, 4 dwg, 3 ex