METHOD FOR PRODUCING OF PROTEIN ISOLATE FROM PROTEINACEOUS SUBSTANCE Russian patent published in 2004 - IPC

FIELD: food-processing industry.

SUBSTANCE: method involves grinding proteinaceous substance into flour; suspending resultant flour in aqueous solution; solving protein of flour with the use of at least one protease introduced in countercurrent flow; settling protein from solution with the use of inorganic acid and neutralizing; transmitting protein into solution by at least double-staged treatment with the use of at least one protease of pH value exceeding 8; heating at temperature of 30-60⁰C; treating flour at first stage with lower concentration of at least one protease at the rate of protein weight, lower pH value and higher temperature than at second stage; after first stage, separating first liquid above sediment from flour-containing fraction; settling protein from said first liquid; after second stage, separating second liquid above sediment from flour-containing fraction and returning second liquid to first stage.

EFFECT: increased protein yield without deteriorating quality of protein isolate.

9 cl, 1 dwg, 2 ex