METHOD OF PRODUCING PROTEIN ISOLATE FROM RAPESEED CAKE Russian patent published in 2024 - IPC A23J1/14 

FIELD: food industry.

SUBSTANCE: invention relates to food industry, namely to methods of rapeseed cake processing in order to obtain rapeseed protein isolate. Method involves grinding rapeseed cake to particle size of not more than 200 mcm, degreasing the obtained rapeseed cake flour with n-hexane at ratio of 1:4–5 at temperature of 40 °C for 1 hour and stirring 180 min^-1, centrifugation at 4,000 min^-1 for 20 minutes, enzymatic hydrolysis of defatted rapeseed meal by preparing an aqueous suspension with a weight fraction of 10–12% defatted flour and setting the pH value of the suspension in range of 5.2–5.5 with help of 10% H₂SO₄ solution, followed by addition of a complex enzyme preparation of cellulase with activity of not less than 2,000 CU/g in dosage 80–90 CU/g of dry raw material, wherein the enzymatic hydrolysis is carried out for 3.5–4 hours at temperature of 50 °C and stirring 180 min^-1, further, alkaline extraction is carried out while bringing the pH of suspension to 8.5–9.0 using 10% NaOH, which is carried out with 0.5 M NaCl solution at temperature of 40 °C for 1 hour and while stirring 180 min^-1, followed by centrifuging suspension at 4,000 min^-1 for 20 minutes to obtain a centrifuge, which is used to precipitate protein by adjusting pH to 4.0–4.2 using 10% HCl solution, wherein precipitated protein is separated at 4,000 min^-1 for 20 minutes, followed by washing with double volume of distilled water and repeated centrifugation at 4,000 min^-1 for 20 minutes, after which the obtained rapeseed protein isolate is dried in a vacuum drying cabinet at temperature of 50 °C and pressure of 100 Pa to residual moisture content of 6–8%.

EFFECT: invention enables to obtain protein isolate with output of 75–80% of total content in raw material, containing 91–92% of target product.

1 cl, 3 ex