METHOD FOR DIFFERENTIAL DIAGNOSIS OF CARCINOGENESIS STAGES Russian patent published in 2004 - IPC

FIELD: medicine, oncology.

SUBSTANCE: method involves histological study of neoplasm tissue slices. Histological slices are studied using computer analyzer and in prepared image small lymphocyte nuclei are isolated, their ploidy is determined and the obtained value is accepted as a standard. Using the latter value the computer determination of an average ploidy of cell nuclei in growing zone of neoplasia is carried out. The normal epithelial tissue or its hyperplasia is diagnosed at value of this index 1.5 s - 2.9 s; benign stage in carcinogenesis - low degree of intraepithelial neoplasia is diagnosed at value of this index 3 s - 3.4 s; boundary stage - high degree of intraepithelial neoplasia is diagnosed at value of this index 3.5 s - 4.4 s, and malignant stage of carcinogenesis - carcinoma is diagnosed at value of this index 4.5 s and above. In diagnosis of carcinoma method involves additional determination of degree of cells de-differentiation. The first highly differentiated stage is diagnosed at values of mean ploidy of cell nuclei 4.5 s - 5.4 s; the second moderately differentiated stage is diagnosed at value 5.5 s - 6.4 s; the third low differentiated stage is diagnosed at value 6.5 s - 7.4 s, and non-differentiated carcinoma is diagnosed at value 7.5 s and above. Method provides the objectivity in carrying out the differential diagnosis.

EFFECT: improved method for diagnosis.

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