FIELD: biotechnology.
SUBSTANCE: invention relates to biotechnology, particularly to genetic engineering. Disclosed is Hansenula polymorpha yeast strain producer of recombinant hepatitis B virus surface antigen serotype "ayw", having mutation G145R (ESC-Ag) (versions). Strain comprises one or several copies of DNA sequence coding mutant antigen ESC-Ag, by promoter gene MOX or strain contains multiple copies of coding sequence by promoter gene MAL1, or strain contains one copy of coding sequence by promoter gene MOX and one copy of coding sequence under control of DAK gene promoter. Strain provides high yield of recombinant protein ESC-Ag with antigenic and immunogenic properties of natural antigen.
EFFECT: invention can be used in microbiological synthesis of recombinant protein ESC-Ag.
4 cl, 7 dwg, 1 tbl, 8 ex
| Title | Year | Author | Number |
|---|---|---|---|
| RECOMBINANT VACCINE FOR PROPHYLAXIS OF HEPATITIS B (VERSIONS) | 2015 |
|
RU2603729C2 |
| RECOMBINANT Hansenula polymorpha YEAST STRAIN - PRODUCER OF HEPATITIS B VIRUS SURFACE ANTIGEN SEROTYPE "ayw" | 2015 |
|
RU2586511C1 |
| METHOD OF OBTAINING A RECOMBINANT VACCINE FOR PREVENTION OF HUMANPAPILLOMA VIRUS INFECTION, RECOMBINANT VACCINE | 2018 |
|
RU2681174C1 |
| RECOMBINANT STRAIN OF YEAST Hansenula polymorpha - PRODUCER OF MAJOR CAPSID PROTEIN L1 OF HUMAN PAPILLOMAVIRUS OF TYPE 56 | 2014 |
|
RU2546240C1 |
| RECOMBINANT STRAIN OF YEAST Hansenula polymorpha - PRODUCER OF MAJOR CAPSID PROTEIN L1 OF HUMAN PAPILLOMAVIRUS OF TYPE 16 | 2014 |
|
RU2546241C1 |
| RECOMBINANT STRAIN OF YEAST Hansenula polymorpha - PRODUCER OF MAJOR CAPSID PROTEIN L1 OF HUMAN PAPILLOMAVIRUS OF TYPE 18 | 2014 |
|
RU2546242C1 |
| RECOMBINANT YEAST STRAIN HARSENULA POLYMORPHA - PRODUCER OF THE MAIN CAPCID PROTEIN L1 OF HUMAN PAPILLOMAVIRUS TYPE 11 | 2018 |
|
RU2676160C1 |
| RECOMBINANT YEAST STRAIN HANSENULA POLYMORPHA PRODUCING THE MAIN CAPSID PROTEIN L1 OF HUMAN PAPILLOMAVIRUS TYPE 6 | 2018 |
|
RU2675471C1 |
| RECOMBINANT STRAIN OF YEAST HANSENULA POLYMORPHA - PRODUCENT OF CAPSID PROTEIN OF HEPATITIS E VIRUS | 2012 |
|
RU2493249C1 |
| RECOMBINANT VACCINE FOR PREVENTION OF VIRAL HEPATITIS E IN ANIMALS | 2012 |
|
RU2501568C1 |
