FIELD: biotechnology, steroids, genetic engineering.
SUBSTANCE: method for preparing ergosterol and its intermediate products is based on using recombinant yeast strains that are able to synthesize enhanced amounts of ergosterol, and plasmid used for transformation of yeast. The species S.cerevisiae is the preferable yeast strain. Expression vectors YepH2, pADL-SAT1 and YDpUHK3 are used as plasmids being each of them comprises the inserted expression cassette comprising the mean ADH1-promoter and terminator TRP. Products formed as result of superexpression can be used for different biotransformation aims.
EFFECT: improved preparing method.
16 cl, 5 tbl, 3 ex
| Title | Year | Author | Number |
|---|---|---|---|
| GENETICALLY MODIFIED ORGANISMS FOR PRODUCTION OF LIPIDS | 2010 |
|
RU2617963C2 |
| METHOD OF PREPARING THE HETEROLOGIC POLYPEPTIDE IN EUCARYOTIC MICROORGANISMS | 1986 |
|
RU2091490C1 |
| INTEGRATIVE PLASMID VECTOR FOR EXPRESSION OF GENES IN YEAST | 2008 |
|
RU2388823C1 |
| METHOD FOR OBTAINING HUMAN @@@-DISMUTASE | 0 |
|
SU1741610A3 |
| RECOMBINANT PLASMID PFS 19 DETERMINING SYNTHESIS OF THE SURFACE ANTIGEN S OF HUMAN VIRUS HEPATITIS B | 1992 |
|
RU2115730C1 |
| STRAIN OF YEAST SACCHAROMYCES CEREVISIAE CONTAINING RECOMBINANT PLASMID YEP 63/AB, - A PRODUCER OF HUMAN HEPATITIS B VIRUS M-PROTEIN DERIVATIVE | 1994 |
|
RU2082759C1 |
| RECOMBINANT PLASMIS DNA pZEN16 FOR TRANSFER AND EXPRESSION OF GENES IN MYCELIAL FUNGUS ACREMONIUM CHRYSOGENUM | 2009 |
|
RU2434944C2 |
| RECOMBINANT PLASMID FOR PHOSPHOLIPASE GENE EXPRESSION IN PICHIA PASTORIS YEAST, PICHIA PASTORIS YEAST STRAIN-PHOSPHOLIPASE PRODUCER | 2009 |
|
RU2409671C1 |
| DOUBLE HYBRID SYSTEM BASED ON GENE SILENCING BY TRANSCRIPTIONAL INTERFERENCE | 2005 |
|
RU2403287C2 |
| RECOMBINANT PLASMID ENCODING HEPATITIS B VIRUS SURFACE ANTIGEN (HBSAG), ITS PREPARING AND YEAST STRAIN HANSENULA POLYMORPHA AS PRODUCER OF HEPATITIS B VIRUS SURFACE ANTIGEN (HBSAG) | 2002 |
|
RU2207374C1 |
