FIELD: biotechnology.
SUBSTANCE: group of inventions relates to biotechnology. Recovered genetically modified organism for producing squalene or squalene derivative is disclosed, in which in comparison with corresponding wild-type organism activity of both paralogs of ARE1 and ARE2 acyl-CoA:sterol acyltransferase/sterol O-acyltransferase (EC 2.3.1.26) is reduced or eliminated, and HMG-CoA reductase activity (EC 1.1.1.34) is increased. Application of said organism for production of lipid is described, which represents squalene or squalene derivative, including cultivation of organism and extraction of lipid from organism.
EFFECT: group of inventions enables to obtain squalene with low content of impurities of not only steril esters, but impurities in whole.
6 cl, 5 dwg, 3 tbl, 8 ex
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