FIELD: biotechnology, genetic engineering, molecular biology, medicine, allergology.
SUBSTANCE: invention relates to DNA recombinant molecule encoding polypeptide-allergen from cereal crops that is expressed by cereal and monocotyledonous plants. Invention describes also nucleic acid fragment encoding immunomodulating T-cellular reactive fragment of polypeptide-allergen. Invention embraces the expression vector and cloning system comprising indicated DNA or its fragment. Invention describes polypeptide-allergen, its fragment and a method for it preparing. Invention represents methods for diagnosis in vivo and in vitro of pollen allergy and features of pollinosis using polypeptide or its fragment and also methods for therapy of humans or animals suffering with pollen allergy by vaccination with DNA vector or administration of pharmaceutical preparation comprising polypeptide or its fragment. Using the invention provides carrying out the specific immunotherapy resulting to change in allergic immune state.
EFFECT: improved preparing method, valuable medicinal properties of allergen.
14 cl, 4 dwg
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