FIELD: medicine, allergology.
SUBSTANCE: invention to a method for isolating and purifying five allergens among groups 1, 2, 3, 10 and 13 from herbal pollen. Method involves preparing an aqueous extract from pollen of plants of family Gramineae. Then soluble components are subjected for hydrophobic chromatography, gel-filtration step and, if necessary, cation-exchange chromatography. Method for diagnosis for pollen in vitro is carried out using obtained allergens.
EFFECT: improved isolating and purifying method.
7 cl, 1 dwg
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