FIELD: chemistry; biochemistry.
SUBSTANCE: present invention relates to genetically modified yeast which independently produces cholesterol from a simple carbon source. The said yeast expresses 7-dehydrocholesterol reductase and 3β-hydroxysterine Δ24-reductase enzymes, while the sterol 24-C-methyltransferase enzyme is inactive. Cholesterol production of the said yeast is equal to or greater than 20% of all produced sterines. The invention also discloses a method of producing cholesterol and using the disclosed yeast strain to produce labelled and non-labelled cholesterol.
EFFECT: invention enables to obtain large amounts of cholesterol which is safe in terms of sanitation in.
12 cl, 13 dwg, 18 ex
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