FIELD: biotechnology.
SUBSTANCE: nucleic acids are extracted from biomass in the presence of hydrogen peroxide in the low concentration as retaining substance. Obtained extract is treated with proteolytic enzyme and RNA is precipitated with acid in the presence of calcium salt. Purification of product is carried out with aqueous solutions of acetone and calcium and sodium chlorides followed by purification of sodium nucleate with an aqueous-acetone solution in the presence of activated carbon. Invention provides elevating yield of the end product and to improve its quality. Invention can be used for preparing sodium nucleate from baking yeast.
EFFECT: improved preparing method.
1 ex
| Title | Year | Author | Number |
|---|---|---|---|
| METHOD OF SODIUM NUCLEINATE PREPARING | 1996 |
|
RU2081915C1 |
| METHOD OF PRODUCING SODIUM NUCLEINATE FROM BIOMASS OF CHLORELLA VULGARIS BEIJERINK MICROALGAE | 2020 |
|
RU2742053C1 |
| METHOD OF PRODUCING SODIUM NUCLEINATE FROM BIOMASS OF CHLORELLA VULGARIS BEIJERINK MICROALGAE | 2020 |
|
RU2742054C1 |
| METHOD FOR SYNTHESISING SODIUM NUCLEINATE FROM CHLORELLA VULGARIS BEIJERINK MICROALGA | 2020 |
|
RU2753608C2 |
| METHOD FOR OBTAINING SODIUM NUCLEINATE FROM DRY BIOMASS OF MICROALGAE CHLORELLA VULGARIS BEIJERINK | 2020 |
|
RU2747120C1 |
| METHOD OF PRODUCING SODIUM NUCLEINATE FROM CHLORELLA VULGARIS BEIJERINK MICROALGAE | 2020 |
|
RU2742056C1 |
| METHOD FOR PRODUCING RIBONUCLEIC ACID | 1996 |
|
RU2103365C1 |
| METHOD FOR PRODUCING DOUBLE-STRANDED RIBONUCLEIC ACID FROM SACCHAROMYCES CEREVISIAE YEAST CELLS | 2022 |
|
RU2781833C1 |
| METHOD OF DEPOSITING NUCLEIC ACIDS FROM SOLUTIONS CONTAINING PHOSPHATE BUFFER | 0 |
|
SU1161511A1 |
| METHOD FOR RIBONUCLEIC ACID PREPARATION FROM THE YEAST CELLS | 0 |
|
SU1708845A1 |
