FIELD: chemistry of natural compounds, polymers.
SUBSTANCE: invention proposes a method for preparing low-molecular heparins from high-molecular heparins by the enzymatic fermentative depolymerization using the enzyme complex from Streptomyces kurssanovii immobilized on silochrome in the weight ratio heparin : immobilized enzyme = 1:1. Then suspension is stirred, the immobilized preparation is separated by centrifugation, washed out and washing liquid is combined with supernatant followed by desalting on column with Sephadex 6-10 and the obtained product is lyophilized.
EFFECT: improved preparing method, reduced cost of method.
2 ex
| Title | Year | Author | Number |
|---|---|---|---|
| METHOD FOR PREPARING HEPARINS OF LOW MOLECULAR MASS | 2005 |
|
RU2295538C2 |
| METHOD FOR PREPARING HEPARIN WITH LOW MOLECULAR WEIGHT AND ANTICOAGULANT ACTIVITY | 2009 |
|
RU2396282C1 |
| METHOD FOR MAKING LOW-MOLECULAR HEPARIN | 2007 |
|
RU2377993C2 |
| METHOD OF PRODUCING ENZYMATIC COMPLEX PREPARATION HAVING COLLAGENASE ACTIVITY | 2008 |
|
RU2412997C2 |
| METHOD OF PRODUCING PAPAIN PREPARATION IN GEL BASED ON FOOD CHITOSAN AND CHITOSAN SUCCINATE | 2019 |
|
RU2712690C1 |
| METHOD OF PRODUCING ANTICOAGULATIVE COLLAGENASE INHIBITOR FROM KING CRAB HEPATOPANCREAS | 2009 |
|
RU2403284C1 |
| METHOD FOR PRODUCING IgAl-PROTEASE FROM SEROGROUP A NEISSERIA MENINGITIDIS AND RELATED IMMUNOGENIC PREPARATION | 2009 |
|
RU2407792C1 |
| METHOD FOR PRODUCING FICIN PREPARATION IN GEL BASED ON CARBOXYMETHYLCELLULOSE | 2021 |
|
RU2771183C1 |
| METHOD OF PREPARING LOW-MOLECULAR CHITOSAN FOR ANTIRADIATION PREPARATIONS | 2000 |
|
RU2188829C1 |
| METHOD OF PRODUCING LOW-MOLECULAR CHITOSAN IN SALT-FREE MEDIUM VIA ENZYMATIC POLYMERISATION | 2009 |
|
RU2425844C2 |
