METHOD OF CULTURING TUBERCULOSIS MYCOBACTERIA Russian patent published in 2005 - IPC

FIELD: medical microbiology.

SUBSTANCE: tuberculosis mycobacteria M. tuberculosis, which can be used for bacteriological diagnostics of tuberculosis, are cultured on enriched Shkolnikova nutrient medium, to which perfluorodecaline substrate is added at 1:1 ratio, which forms lower phase to improve gas exchange and enhancing growth of mycobacteria. Concentration of free ions in perfluorodecaline substrate is below 10^-6 and amount of incompletely fluorinated admixtures below 0.003 wt %. Biphasic medium for culturing mycobacteria is prepared by placing 2 ml sterile perfluorodecaline into test tube followed by placing 2 ml Shkolnikova medium over it to form system, wherein lower phase occupies heavier perfluorodecaline and higher phase nutritive broth. Culturing of virulent laboratory strain H37Rv on above medium revealed considerable increase in growth of mycobacteria compared to conventional Shkolnikova medium. On the 7^th day after inoculation, amount of M. tuberculosis in biphasic medium was superior to that in conventional Shkolnikova medium by a factor of 1.7 and on the 10^th day by a factor of 3.27.

EFFECT: enhanced growth of mycobacteria.

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