METHOD FOR IN VITRO PREPARING LAWN GRASS WITH TOLERANCE TO SALINIZATION Russian patent published in 2005 - IPC

Abstract RU 2260936 C1

FIELD: biotechnology and cellular engineering.

SUBSTANCE: invention relates to a method for preparing salt-resistant plants. Method involves culturing fescue and bent-grass lawn grasses up to preparing callus in Murasige-Skuga medium with 2,4-dichlorophenoxyacetic acid in the concentration from 3 to 10 mg/l followed by passage of callus for 2-4 passages in Murasige-Skuga medium with 2,4-dichlorophenoxyacetic acid in the concentration 1-3 mg/l and 1% sodium chloride up to preparing regenerating calluses. Then method involves regeneration and rooting plants in Murasige-Skuga medium with 1% of sodium chloride. Invention provides rapid and effective preparing plants of fescue (Festuca) and bent-grass (Agrostis) with the stable and inherited resistance against the salinization factor.

EFFECT: improved preparing method.

3 tbl, 3 ex

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RU 2 260 936 C1

Authors

Gladkov E.A.

Dolgikh Ju.I.

Birjukov V.V.

Gladkova O.V.

Shevjakova N.I.

Gladkova O.N.

Glushetskaja L.S.

Dates

2005-09-27Published

2004-02-12Filed