METHOD OF INTRODUCTION IN CULTURE OF PERENNIAL FLAX CELLS Russian patent published in 2014 - IPC A01H4/00 

FIELD: agriculture.

SUBSTANCE: invention relates to the field of biotechnology. The invention is obtaining of tylosis on the Murashige and Skoog nutrient medium made up to 1 litre with water, during one passage, where in the nutrient medium 2,4-dichlorophenoxyacetic acid is added at concentrations of 4-8 mg/l, the perennial flax cells are cultivated, in the Gamborg nutrient medium 6-benzylaminopurine is added at a concentration of 1-2 mg/l and α-naphthylacetic acid at a concentration of 0.1-0.5 mg/l and then on the Gamborg nutrient medium the obtained tylosis are transplanted for regeneration and cultivated for 2-4 passages, in a liquid Murashige and Skoog nutrient medium at a concentration of 50% of all the components α-naphthylacetic acid is added at a concentration of 0.1-0.5 mg/l and regenerated plants are transplanted on it and are cultivated for 2-4 passages.

EFFECT: invention enables to introduce into the culture of perennial flax cells.

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