METHOD FOR ORGANS AND TISSUES CRYOCONSERVATION IN SITU Russian patent published in 2006 - IPC A01N1/02 

FIELD: medicine.

SUBSTANCE: biological object (heart, kidney, etc) should be cooled in water up to 0° C at simultaneous saturation with the mixture of xenon, krypton, argon at the ratio of 2.5:47.5:50 rot.%, then one should force out water with gaseous mixture mentioned and at the pressure of 1.5 atm. it is necessary to decrease temperature up to -43° C, then one should decrease the pressure of gaseous medium up to normal level and continue cooling up to -196° C. The innovation enables to achieve reliable and prolonged cryoconservation of animal heart being the constituent of the whole organism (in situ), in a transferred transplant conserved for 6 h against the time of cold cardioplegia and up to the moment of restoring contractile activity. Moreover, it has been possible to achieve restoration of adequate cardiac activity.

EFFECT: higher efficiency.

1 dwg, 1 ex