METHOD FOR PRODUCTION OF CONDITIONING MEDIUM HAVING COLONY-PROMOTING ACTIVITY Russian patent published in 2006 - IPC A61K35/28 C12N5/06 

FIELD: medicine, in particular hematology.

SUBSTANCE: CBA/CaLac intact mouse spleen is homogenized in medium containing 95 % of RPMI-1640 and 5 % of ethyl silicate; filtered through nylon grid; washed by centrifugation with RPMI-1640. at 1500 rpm for 10-15 min; final concentration of living splenocites is adjusted to 2x10⁶ colony/ml using media containing 10 % of ethyl silicate, 1 % of L-glutamine; 0.2 % of gentamycine, 0.05 % of mercaptoethanol, 89 % of RPMI-1640; obtained product is cultivated at 37°C for 6 days, centrifuged and supernatant containing target product is separated followed by storage at -20°C. Before filtering 1 ml of distilled water is added in sterile spleen homogenate and after 25 sec 10 ml of RPMI-1640 medium is added. Target product is stored for 1 month or less.

EFFECT: method of improved accuracy and validity.

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