METHOD FOR STIMULATING GRANULOCYTE COLONY STIMULATING FACTOR PRODUCTION BY BONE MARROW CELLS IN VITRO Russian patent published in 2018 - IPC C12N5/00 

FIELD: biotechnology.

SUBSTANCE: invention relates to biotechnology. Invention is a method for stimulating production of granulocyte colony stimulating factor by bone marrow cells in vitro, consisting in fact that bone marrow cells are cultured in CO₂-incubator at 37 °C, 5 % CO₂ and 100 % air humidity for 24 hours in a complete culture medium of the following composition: 90 % of the RPMI-1640 medium, 10 % EF, 280 mg/l of L-glutamine, 50 mg/l gentamicin with the addition of a stimulant, where a non-stick fraction of bone marrow cells is cultured, and a PI3K inhibitor at a concentration of 50 mcM is used as a stimulant.

EFFECT: proposed method allows to effectively stimulate production of granulocyte colony-stimulating factor by bone marrow cells in vitro.

1 cl, 1 tbl, 1 ex