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2 589 288

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IPC

A61K35/12(2015-01-01)

C12N5/71(2010-01-01)

C12N9/99(2006-01-01)

C07K14/505(2006-01-01)

(21) (22)

Application

2015121490/15, 2015-06-04

(24)

Start date

2015-06-04

(22)

Actual filing date

2015-06-04

(45)

Published

2016-07-10

(72)

Inventor

Dygaj Aleksandr MikhajlovichZhdanov Vadim VadimovichZyuzkov Gleb NikolaevichMiroshnichenko Larisa ArkadevnaUdut Elena VladimirovnaKhrichkova Tatyana YurevnaSimanina Elena VladislavovnaSherstoboev Evgenij YurevichAgafonov Vladimir IvanovichBurmina Yana VadimovnaMinakova Mariya Yurevna

(73)

Holder

Federalnoe Gosudarstvennoe Byudzhetnoe Nauchnoe Uchrezhdenie Institut Farmakologii I Regenerativnoj Meditsiny Imeni E.D. Goldberga"

METHOD FOR STIMULATING PRODUCTION OF ERYTHROPOIETIN IN A CELL CULTURE in vitro Russian patent published in 2016 - IPC A61K35/12 C12N5/71 C12N9/99 C07K14/505

Abstract RU 2589288 C1

FIELD: medicine.

SUBSTANCE: invention relates to experimental biology and medicine, specifically to a method for erythropoietin production stimulation of bone marrow cells in vitro. Method for stimulating production of erythropoietin bone marrow cells in vitro, consists in that non-adhesive fraction of myelocytes is cultivated in a CO₂-incubator in a complete culture medium of following composition: 90 % RPMI-1640 medium, 10 % ETS, 280 mg/l L-glutamine, 50 mg/l gentamycin, addition of inhibitor of protein kinase p38 in concentration 300 mcM under certain conditions.

EFFECT: method described above enables to efficiently stimulate development of erythropoietin with non-adhesive myelocariocytes in vitro.

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RU 2 589 288 C1

Authors

Dygaj Aleksandr Mikhajlovich

Zhdanov Vadim Vadimovich

Zyuzkov Gleb Nikolaevich

Miroshnichenko Larisa Arkadevna

Udut Elena Vladimirovna

Khrichkova Tatyana Yurevna

Simanina Elena Vladislavovna

Sherstoboev Evgenij Yurevich

Agafonov Vladimir Ivanovich

Burmina Yana Vadimovna

Minakova Mariya Yurevna

Dates

2016-07-10—Published

2015-06-04—Filed