METHOD FOR PREPARING L-AMINO ACIDS FROM RACEMIC N-ACETYL-D,L-DERIVATIVES BY ENZYMATIC CLEAVAGE Russian patent published in 2006 - IPC C12P41/00 C12P13/04 C12N15/55 

FIELD: biotechnology, amino acids, biochemistry.

SUBSTANCE: invention relates to a method for preparing proteinogenic or non-proteinogenic L-amino acids, especially L-phosphotricine, from their racemic N-acetyl-D,L-derivatives. Method involves enzymatic cleavage of racemate by using recombinant hyppurate hydrolases that results to selective deacetylation of N-acetyl-L-derivatives of corresponding L-amino acids while N-acetyl-D-derivatives of corresponding D-amino acids are not deacetylated. Prepared deacetylated L-amino acids are separated by preparative method from non-deacetylated N-acetyl-D-derivatives and/or not fully deacetylated N-acetyl-L-derivatives. Invention provides preparing L-amino acids with the high yield and purity of enantiomers.

EFFECT: improved preparing method.

9 cl, 4 tbl, 6 ex