FIELD: chemistry.
SUBSTANCE: invention relates to biochemistry, in particular to method of obtaining product of reaction, which is catalysed by nitrolase, fused with transport domain of autotransporter, where method includes the following stages: (i) provision of Gram-negative bacterium, containing said nitrilase, located on its surface, and/or membrane preparation of said Gram-negative bacterium, and (ii) bringing Gram-negative bacterium and/or its membrane preparation in contact with one or more nitrilase substrates under conditions, compatible with nitrilase activity.
EFFECT: invention makes it possible to increase nitrilase stability and increase output of reaction product.
17 cl, 18 dwg, 3 ex
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