METHOD FOR PREPARING ENZYME PREPARATION FROM CRAB HEPATOPANCREAS Russian patent published in 2006 - IPC C12N9/00 C12N9/50 C12N9/64 

FIELD: biotechnology.

SUBSTANCE: method involves isolation of enzyme from crab frozen hepatopancreas by autolysis. Autolysis is carried out in deionized water solution at temperature 21°C, not above, in the ratio crab hepatopancreas : deionized water = 1:4, respectively, and at periodic stirring for 14-15 h. Prepared mixture is filtered at temperature 21°C under pressure through a sieve with cells size 5 mm and then through filtering devices with cells size 1.5 and 0.1 mm, respectively. Then sodium hydrocarbonate solution is added to the prepared filtrate and stirred for 5 min followed by addition of chitosan ascorbate solution at temperature 21°C, not above, to the prepared mixture in the total parts ratio filtrate : sodium hydrocarbonate solution : chitosan ascorbate solution = 1.25:0.15:1, respectively. Mixture is stirred for 10-15 min, kept for 1.5 h up to separating into layers for protein and lipid fractions and protein fraction is fed to successive microfiltration and then to ultrafiltration with cooling filtrate to 12°C. Ultrafiltration is carried out in closed-type cycle at feeding deionized water and maintenance the ratio value of protein solution and deionized water = 2:1. Invention provides simplifying the process for preparing the enzyme preparation and the possibility for its easy automatization and enhancing ecology effectiveness of the process. Invention can be used in perfume-cosmetic industry, leather and bellows manufacture and in chemical-pharmaceutical industry.

EFFECT: improved preparing method.

2 cl, 1 ex