FIELD: microbiology, biotechnology.
SUBSTANCE: bird with body mass of 300-500 g is inoculated on high air passage mucous and after death its dead body cools down at environmental temperature (preferably at 20°C) for 3 hours. Then dead body is dissected and heart blood is sampled. Pasteurellas are detected in blood in form of bipolar uniformly formed pseudospatula in state of frank capsulation and immediately isolated from nutritive broth into physiological solution (pH 7.0). Pasteurellas are stored for 10 days without accession of atmospheric air in filled, sealed and horizontally arranged Pasteur pipettes at 20°C in form of morphologically identical capsulated coccobacillus. Pasteurellas which are stored for application are seeded at dilution of 10-1 - 10-10 into liquid broth with the same temperature (20°C), cultured for 9 h at 37°C and for 1 h at 20°C. Standard pasteurella culture obtained at minimum amount of microbial cell in seeding is titrated on bird of predetermined genus and age and minimum fatal dose in 0.5 ml or 1.0 ml is determined. Birds are inoculated by intranasal administration.
EFFECT: high accurate method to render natural conditions of bird inoculation with pasteurellosis excitant.
2 tbl, 6 ex
